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DNA
Phosphoamidites & CPG's
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DNA Phosphoramidites
Analytical Specifications of DNA Phosphoramidites
| Test |
dA(bz)
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dC(bz)
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dG(ib)
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dT
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| 31P-NMR |
> 99%
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> 99%
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> 99%
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> 99%
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| RP-HPLC |
> 99.0%
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> 99.0%
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> 99.0%
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> 99.0%
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Coupling
efficiency |
> 99.0%
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> 98.0%
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> 99.0%
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> 99.0%
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Key Features of DNA Phosphoramidites
 | Exocyclic amine functions are protected by a benzoyl group (dA(bz)
and dC(bz)) or isobutyryl group (dG(ib)). |
| Recommended cleavage and deprotection conditions are 8 hours at 55°C
or 24 hours at room temperature using concentrated ammonia solution,
for standard base-protected oligonucleotides. |
| The high coupling efficiency of Proligo Reagents' DNA
phosphoramidites leads to high-yield, high-quality oligonucleotides. |
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%20Phosphoramidite.gif)
dA(bz) Phosphoramidite
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%20Phosphoramidite.gif)
dC(bz) Phosphoramidite |
%20Phosphoramidite.gif)
dG(ib) Phosphoramidite |
dT Phosphoramidite |
Fast Deprotection DNA amidites
DMT-dA(tac)
amidite
DMT-dC(tac)
amidite
DMT-dG(tac)
amidite
DMT-dG(dmf) amidite
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Controlled Pore Glass and Columns
Since Merrifield first described solid phase synthesis, researchers have
investigated numerous supports for the chemical synthesis of DNA.
Controlled Pore Glass (CPG) has proven to be the most accepted standard,
with features such as high surface area, efficient mass transfer and
chemical inertness.
Because the pore density of CPG can be adjusted, the solid support can be
tailored to the size of the synthetic oligonucleotide required. Bigger
pore size is typically preferable in minimizing steric effects during the
synthesis of long oligonucleotides, while smaller pore size fosters
improved synthesis consistency.
Our process guarantees consistent production of CPGs to specifications
designed for DNA synthesis. All products are tested in our Quality Control
labs for coupling efficiency and base purity, prior to release. Proligo
Reagent's facility in Hamburg, Germany is ISO 9001 certified.
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Analytical Parameters of CPG
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CPG UHL*
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CPG 500Å
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CPG 1000Å
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| Pore size (nm) |
33
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50
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100
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| Pore volume (ml/g) |
1.3
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1.0
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1.3
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| Grain size (µm) |
100 - 180
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100 - 180
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100 - 180
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| Surface (m2/g) |
~100
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~60
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~30
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*Ultra-high load (UHL)
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Ultra-High-Load (UHL)
Proligo Reagent's Ultra-High-Load (UHL) CPG offers higher
nucleoside-loading than standard CPG, as well as smaller pore-size for
special applications.
Nucleoside-loaded CPG
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CPG 500Å
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Loading µmol/g
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30-40
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CPG 1000Å
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25-35
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CPG UHL
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50-70
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CPG UHL
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90-110
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DNA Phosphoramidites
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PE 8900 and Polygen compatible
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1gx12
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2gx12
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dA(bz)
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A111081-12
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A111082-12
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dC(bz)
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C111081-12
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C111082-12
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dG(ib)
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G111081-12
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G111082-12
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dT
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T111081-12
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T111082-12
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1oz
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2oz
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20/400 screw-cap bottle
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MerMade compatible
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10gx1
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10gx6
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dA(bz)
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A111028-01
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A111028-06
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dC(bz)
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C111028-01
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C111028-06
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dG(ib)
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G111028-01
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G111028-06
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dT
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T111028-01
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T111028-06
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8oz
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8oz
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28/400 screw-cap bottle
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| Please enquire about our Starter Kits. |
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ABI compatible
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1gx12
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2gx12
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dA(bz)
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A111031-12
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A111032-12
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dC(bz)
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C111031-12
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C111032-12
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dG(ib)
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G111031-12
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G111032-12
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dT
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T111031-12
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T111032-12
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15ml
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60ml
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septum bottle
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ABI 3900 (high-throughput) compatible
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2gx12
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dA(bz)
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A111032-12
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dC(bz)
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C111032-12
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dG(ib)
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G111032-12
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dT
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T111032-12
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60ml
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septum bottle
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Bulk DNA Phosphoramidites
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5gx1
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10gx1
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20gx6
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100gx1
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dA(bz)
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A111005-01
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A111010-01
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A111020-06
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A111011-01
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dC(bz)
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C111005-01
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C111010-01
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C111020-06
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C111011-01
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dG(ib)
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G111005-01
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G111010-01
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G111020-06
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G111011-01
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dT
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T111005-01
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T111010-01
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T111020-06
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T111011-01
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100ml
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100ml
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16oz
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500ml
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septum bottle
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28/400 screw-cap bottle
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Controlled Pore Glass and Columns
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PE 8900 compatible
CPG columns
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CPG 500Åx100
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50nmol
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0.2µmol
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1µmol
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dA(bz)
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A311010-01
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A321010-01
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A331010-01
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dC(bz)
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C311010-01
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C321010-01
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C331010-01
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dG(ib)
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G311010-01
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G321010-01
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G331010-01
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dT
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T311010-01
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T321010-01
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T331010-01
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PE 8900 compatible
CPG columns
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CPG 1000Åx100
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0.2µmol
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1µmol
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dA(bz)
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A421010-01
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A431010-01
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dC(bz)
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C421010-01
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C431010-01
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dG(ib)
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G421010-01
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G431010-01
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dT
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T421010-01
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T431010-01
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Bulk CPG
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500Å loading 30-40µmol/g
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1000Å loading
25-35µmol/g
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1g
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10g
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1g
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10g
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dA(bz)
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A301001-01
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A301010-01
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A401001-01
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A401010-01
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dC(bz)
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C301001-01
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C301010-01
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C401001-01
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C401010-01
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dG(ib)
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G301001-01
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G301010-01
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G401001-01
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G401010-01
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dT
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T301001-01
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T301010-01
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T401001-01
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T401010-01
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Ultra-high load CPG
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Loading 50-70µmol/g
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Loading 90-110µmol/g
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1g
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10g
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10g
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dA(bz)
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A503001-01
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A503010-01
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A501010-01
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dC(bz)
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C503001-01
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C503010-01
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C501010-01
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dG(ib)
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G503001-01
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G503010-01
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G501010-01
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dT
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T503001-01
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T503010-01
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T501010-01
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Custom Products
Please enquire for custom DNA phosphoramidites, CPG and columns.
Ohter quantities are available upon request.
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