DNA Amidites & CPG

快興科技股份有限公司

DNA Amidites & CPG

DNA Phosphoamidites & CPG's

DNA Phosphoramidites

Analytical Specifications of DNA Phosphoramidites

Test	dA(bz)	dC(bz)	dG(ib)	dT
³¹P-NMR	> 99%	> 99%	> 99%	> 99%
RP-HPLC	> 99.0%	> 99.0%	> 99.0%	> 99.0%
Coupling efficiency	> 99.0%	> 98.0%	> 99.0%	> 99.0%

Key Features of DNA Phosphoramidites

Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)).
Recommended cleavage and deprotection conditions are 8 hours at 55°C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides.
The high coupling efficiency of Proligo Reagents' DNA phosphoramidites leads to high-yield, high-quality oligonucleotides.

dA(bz) Phosphoramidite

dC(bz) Phosphoramidite

dG(ib) Phosphoramidite

dT Phosphoramidite

Fast Deprotection DNA amidites

DMT-dA(tac) amidite

DMT-dC(tac) amidite

DMT-dG(tac) amidite

DMT-dG(dmf) amidite

Controlled Pore Glass and Columns

Since Merrifield first described solid phase synthesis, researchers have investigated numerous supports for the chemical synthesis of DNA. Controlled Pore Glass (CPG) has proven to be the most accepted standard, with features such as high surface area, efficient mass transfer and chemical inertness.
Because the pore density of CPG can be adjusted, the solid support can be tailored to the size of the synthetic oligonucleotide required. Bigger pore size is typically preferable in minimizing steric effects during the synthesis of long oligonucleotides, while smaller pore size fosters improved synthesis consistency.
Our process guarantees consistent production of CPGs to specifications designed for DNA synthesis. All products are tested in our Quality Control labs for coupling efficiency and base purity, prior to release. Proligo Reagent's facility in Hamburg, Germany is ISO 9001 certified.

Analytical Parameters of CPG

CPG UHL*	CPG 500Å	CPG 1000Å
Pore size (nm)	33	50	100
Pore volume (ml/g)	1.3	1.0	1.3
Grain size (µm)	100 - 180	100 - 180	100 - 180
Surface (m²/g)	~100	~60	~30

*Ultra-high load (UHL)

Ultra-High-Load (UHL)
Proligo Reagent's Ultra-High-Load (UHL) CPG offers higher nucleoside-loading than standard CPG, as well as smaller pore-size for special applications.

Nucleoside-loaded CPG

CPG 500Å	Loading µmol/g	30-40
CPG 1000Å		25-35
CPG UHL		50-70
CPG UHL		90-110

Product List for DNA Phosphoramidites, CPG and Columns

DNA Phosphoramidites

PE 8900 and Polygen compatible
	1gx12	2gx12
dA(bz)	A111081-12	A111082-12
dC(bz)	C111081-12	C111082-12
dG(ib)	G111081-12	G111082-12
dT	T111081-12	T111082-12
	1oz	2oz
	20/400 screw-cap bottle

MerMade compatible
	10gx1	10gx6
dA(bz)	A111028-01	A111028-06
dC(bz)	C111028-01	C111028-06
dG(ib)	G111028-01	G111028-06
dT	T111028-01	T111028-06
	8oz	8oz
	28/400 screw-cap bottle
Please enquire about our Starter Kits.

ABI compatible
	1gx12	2gx12
dA(bz)	A111031-12	A111032-12
dC(bz)	C111031-12	C111032-12
dG(ib)	G111031-12	G111032-12
dT	T111031-12	T111032-12
	15ml	60ml
	septum bottle

ABI 3900 (high-throughput) compatible
	2gx12
dA(bz)	A111032-12
dC(bz)	C111032-12
dG(ib)	G111032-12
dT	T111032-12
	60ml
	septum bottle

Bulk DNA Phosphoramidites
	5gx1	10gx1	20gx6	100gx1
dA(bz)	A111005-01	A111010-01	A111020-06	A111011-01
dC(bz)	C111005-01	C111010-01	C111020-06	C111011-01
dG(ib)	G111005-01	G111010-01	G111020-06	G111011-01
dT	T111005-01	T111010-01	T111020-06	T111011-01
	100ml	100ml	16oz	500ml
	septum bottle		28/400 screw-cap bottle

Controlled Pore Glass and Columns

PE 8900 compatible CPG columns
	CPG 500Åx100
	50nmol	0.2µmol	1µmol
dA(bz)	A311010-01	A321010-01	A331010-01
dC(bz)	C311010-01	C321010-01	C331010-01
dG(ib)	G311010-01	G321010-01	G331010-01
dT	T311010-01	T321010-01	T331010-01

PE 8900 compatible CPG columns
	CPG 1000Åx100
	0.2µmol	1µmol
dA(bz)	A421010-01	A431010-01
dC(bz)	C421010-01	C431010-01
dG(ib)	G421010-01	G431010-01
dT	T421010-01	T431010-01

Bulk CPG
	500Å loading 30-40µmol/g		1000Å loading 25-35µmol/g
	1g	10g	1g	10g
dA(bz)	A301001-01	A301010-01	A401001-01	A401010-01
dC(bz)	C301001-01	C301010-01	C401001-01	C401010-01
dG(ib)	G301001-01	G301010-01	G401001-01	G401010-01
dT	T301001-01	T301010-01	T401001-01	T401010-01

Ultra-high load CPG
	Loading 50-70µmol/g		Loading 90-110µmol/g
	1g	10g	10g
dA(bz)	A503001-01	A503010-01	A501010-01
dC(bz)	C503001-01	C503010-01	C501010-01
dG(ib)	G503001-01	G503010-01	G501010-01
dT	T503001-01	T503010-01	T501010-01

Custom Products

Please enquire for custom DNA phosphoramidites, CPG and columns.

Ohter quantities are available upon request.