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Substitution of standard protecting groups with the labile TAC TAC (tert.butylphenoxyacetyl)
protecting group results in ultra-fast and easy deprotection under mild
conditions, suitable for oligonucleotides with base-labile monomers and
reporters as well as in-situ synthesis schemes on glass surfaces.
Key Features of TAC Chemistry
- Deprotection of the TAC group is ultra-fast: complete deprotection
in concentrated ammonia occurs within 15 minutes at 55°C or two hours
at room temperature.
- TAC protecting groups are compatible with the AMA deprotection
reagent.
- TAC protected amidites are highly soluble in acetonitrile. There is
no need to add co-solvents such as dimethylformamide or methylene
chloride.
- TAC monomers are suitable for the synthesis of oligomers with
base-labile units e.g. dyes and modifiers, because of less exposure to
ammonia and the possibility of room temperature deprotection.
- No change is required in the reagents commonly used for DNA
synthesis, except that Fast Deprotection CAP A solution is used
instead of Cap A solution.
- The application of dA(tac) minimizes depurination and improves the
quality of oligonucleotides.
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Changing the dG protection group to the dimethylformamidine (dmf)
base-protecting group enables rapid synthesis of high-purity, high-yield
oligonucleotides, thus increasing the efficiency of high-throughput
production.
Key Features of dG(dmf)
- dG(dmf) is deprotected faster than the conventional dG(ib): the
deprotection time in concentrated ammonia is reduced to 2 hours at 55°C
or 1 hour at 65°C.
- The dG(dmf)-monomer is especially suitable for G-rich sequences:
incomplete deprotection is greatly reduced in comparison with the
conventional dG(ib)-monomer.
- dG(dmf)-amidite is as stable in solution as the standard dA(bz)-,
dC(bz)- and dT-amidites.
- dG(dmf)-amidite can directly substitute for dG(ib)-amidite.
- No change is required in the reagents commonly used for DNA
synthesis (except a low concentration iodine oxidizer i.e. 0.02M in
iodine, should be employed).
%20Phosphoramidite.gif)
dG(dmf) Phosphoramidite |
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Changing the dC protecting group to the TAC protecting group leads to
rapid synthesis of high-purity and high-yield oligonucleotides.
Substituting the commonly employed dC(bz) monomer by the dC(tac) monomer
enables the application of ultra-fast deprotection with the AMA reagent
and provides a high-throughput method of oligonucleotide synthesis.
Key Features of Substituting the dCProtecting Group
- The deprotection of oligonucleotide synthesis products with the AMA
reagent is ultra-fast: complete deprotection requires 10 minutes at 65°C.
- Side reactions at C-monomers through transamination are eliminated.
- Not compatible with some base-labile modified nucleosides.
- dC(tac)-amidite can directly substitute for dC(bz)-amidite.
- No change is required in the reagents commonly used for DNA
synthesis: acetonitrile is used to dissolve the amidite. The standard
acetic anhydride capping reagent can be employed.
%20Phosphoramidite.gif)
dC(tac) Phosphoramidite
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1. TAC Chemistry
DNA Phosphoramidites
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PE 8900 and Polygen compatible
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1gx1
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2gx1
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dA(tac)
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A112081-01
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A112082-01
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dC(tac)
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C112081-01
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C112082-01
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dG(tac)
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G112081-01
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G112082-01
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1gx12
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2gx12
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dA(tac)
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A112081-12
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A112082-12
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dC(tac)
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C112081-12
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C112082-12
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dG(tac)
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G112081-12
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G112082-12
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1oz
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2oz
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20/400 screw-cap bottle
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ABI compatible
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1gx1
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2gx1
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dA(tac)
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A112031-01
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A112032-01
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dC(tac)
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C112031-01
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C112032-01
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dG(tac)
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G112031-01
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G112032-01
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1gx12
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2gx12
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dA(tac)
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A112031-12
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A112032-12
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dC(tac)
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C112031-12
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C112032-12
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dG(tac)
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G112031-12
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G112032-12
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15ml
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60ml
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septum bottle
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For bulk quantities please enquire.
Liquid Reagents
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PE 8900 and Polygen compatible
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Fast Deprotection Cap A
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200mlx6
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L870020-06
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ABI compatible
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Fast Deprotection Cap A
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180mlx6
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L370018-06
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Note: For our entire range of liquid reagents for oligonucleotide
synthesis, please refer to our Liquid Reagents brochure.
For bulk quantities please enquire.
Controlled Pore Glass and Columns
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PE 8900 and Polygen compatible
CPG columns
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CPG 500Åx4
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50nmol
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0.2µmol
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1µmol
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dA(tac)
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A312004-01
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A322004-01
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A332004-01
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dC(tac)
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C312004-01
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C322004-01
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C332004-01
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dG(tac)
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G312004-01
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G322004-01
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G332004-01
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CPG 1000Åx4
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dA(tac)
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-
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-
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A432004-01
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dC(tac)
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-
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-
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C432004-01
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dG(tac)
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-
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-
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G432004-01
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Bulk CPG
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500Å loading 30-40µmol/g
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1g
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dA(tac)
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A302001-01
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dC(tac)
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C302001-01
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dG(tac)
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G302001-01
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1000Å loading 25-35µmol/g
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dA(tac)
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A402001-01
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dC(tac)
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C402001-01
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dG(tac)
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G402001-01
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2. dG(dmf) Method
DNA Phosphoramidites
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PE 8900 and Polygen compatible
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1gx12
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2gx12
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dG(dmf)
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G115081-12
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G115082-12
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1oz
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2oz
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20/400 screw-cap bottle
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ABI compatible
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1gx12
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2gx12
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dG(dmf)
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G115031-12
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G115032-12
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15ml
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60ml
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septum bottle
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For bulk quantities please enquire.
Controlled Pore Glass
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Bulk CPG
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500Å loading 30-40µmol/g
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1g
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10g
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dG(dmf)
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G305001-01
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G305010-01
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3. Substituting the dC Protecting Group
DNA Phosphoramidites
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PE8900 and Polygen compatible
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1gx1
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2gx1
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dC(tac)
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C112081-01
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C112082-01
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1gx12
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2gx12
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dC(tac)
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C112081-12
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C112082-12
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1oz
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2oz
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20/400 screw-cap bottle
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ABI compatible
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1gx1
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2gx1
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dC(tac)
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C112031-01
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C112032-01
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1gx12
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2gx12
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dC(tac)
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C112031-12
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C112032-12
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15ml
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60ml
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septum bottle
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For bulk quantities please enquire.
Controlled Pore Glass
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Bulk CPG
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500Å loading
30-40µmol/g
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1000Å loading
25-35µmol/g
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1g
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1g
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dC(tac)
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C302001-01
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dC(tac)
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C402001-01
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Custom Products
Please enquire for custom DNA Phosphoramidites, CPG and Columns.
Ohter quantities are available upon request.
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