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                                           Fast Deprotection Chemistry

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TAC Chemistry (use fast deprotection Cap A)

　

Substitution of standard protecting groups with the labile TAC TAC (tert.butylphenoxyacetyl) protecting group results in ultra-fast and easy deprotection under mild conditions, suitable for oligonucleotides with base-labile monomers and reporters as well as in-situ synthesis schemes on glass surfaces.

Key Features of TAC Chemistry
bulletDeprotection of the TAC group is ultra-fast: complete deprotection in concentrated ammonia occurs within 15 minutes at 55°C or two hours at room temperature.
bulletTAC protecting groups are compatible with the AMA deprotection reagent.
bulletTAC protected amidites are highly soluble in acetonitrile. There is no need to add co-solvents such as dimethylformamide or methylene chloride.
bulletTAC monomers are suitable for the synthesis of oligomers with base-labile units e.g. dyes and modifiers, because of less exposure to ammonia and the possibility of room temperature deprotection.
bulletNo change is required in the reagents commonly used for DNA synthesis, except that Fast Deprotection CAP A solution is used instead of Cap A solution.
bulletThe application of dA(tac) minimizes depurination and improves the quality of oligonucleotides.

　

　

dG(dmf) Method

　

Changing the dG protection group to the dimethylformamidine (dmf) base-protecting group enables rapid synthesis of high-purity, high-yield oligonucleotides, thus increasing the efficiency of high-throughput production.


Key Features of dG(dmf)

bulletdG(dmf) is deprotected faster than the conventional dG(ib): the deprotection time in concentrated ammonia is reduced to 2 hours at 55°C or 1 hour at 65°C.
bulletThe dG(dmf)-monomer is especially suitable for G-rich sequences: incomplete deprotection is greatly reduced in comparison with the conventional dG(ib)-monomer.
bulletdG(dmf)-amidite is as stable in solution as the standard dA(bz)-, dC(bz)- and dT-amidites.
bulletdG(dmf)-amidite can directly substitute for dG(ib)-amidite.
bulletNo change is required in the reagents commonly used for DNA synthesis (except a low concentration iodine oxidizer i.e. 0.02M in iodine, should be employed).

          dG(dmf) Phosphoramidite

　

Subsituting the dC Protecting Group

　

Changing the dC protecting group to the TAC protecting group leads to rapid synthesis of high-purity and high-yield oligonucleotides. Substituting the commonly employed dC(bz) monomer by the dC(tac) monomer enables the application of ultra-fast deprotection with the AMA reagent and provides a high-throughput method of oligonucleotide synthesis.

Key Features of Substituting the dCProtecting Group

bulletThe deprotection of oligonucleotide synthesis products with the AMA reagent is ultra-fast: complete deprotection requires 10 minutes at 65°C.
bulletSide reactions at C-monomers through transamination are eliminated.
bulletNot compatible with some base-labile modified nucleosides.
bulletdC(tac)-amidite can directly substitute for dC(bz)-amidite.
bulletNo change is required in the reagents commonly used for DNA synthesis: acetonitrile is used to dissolve the amidite. The standard acetic anhydride capping reagent can be employed.

          dC(tac) Phosphoramidite

　

Product List for Fast Deprotection Chemistries

　

1. TAC Chemistry

DNA Phosphoramidites
PE 8900 and Polygen compatible
1gx1
2gx1
dA(tac)
A112081-01
A112082-01
dC(tac)
C112081-01
C112082-01
dG(tac)
G112081-01
G112082-01
1gx12
2gx12
dA(tac)
A112081-12
A112082-12
dC(tac)
C112081-12
C112082-12
dG(tac)
G112081-12
G112082-12
1oz
2oz
20/400 screw-cap bottle
                                                                                   
ABI compatible
1gx1
2gx1
dA(tac)
A112031-01
A112032-01
dC(tac)
C112031-01
C112032-01
dG(tac)
G112031-01
G112032-01
1gx12
2gx12
dA(tac)
A112031-12
A112032-12
dC(tac)
C112031-12
C112032-12
dG(tac)
G112031-12
G112032-12
15ml
60ml
septum bottle

For bulk quantities please enquire.


Liquid Reagents
PE 8900 and Polygen compatible
Fast Deprotection Cap A
200mlx6
L870020-06

ABI compatible
Fast Deprotection Cap A
180mlx6
L370018-06

Note: For our entire range of liquid reagents for oligonucleotide synthesis, please refer to our Liquid Reagents brochure.

For bulk quantities please enquire.


Controlled Pore Glass and Columns
PE 8900 and Polygen compatible
CPG columns
CPG 500Åx4
50nmol
0.2µmol
1µmol
dA(tac)
A312004-01
A322004-01
A332004-01
dC(tac)
C312004-01
C322004-01
C332004-01
dG(tac)
G312004-01
G322004-01
G332004-01
CPG 1000Åx4
dA(tac)
-
-
A432004-01
dC(tac)
-
-
C432004-01
dG(tac)
-
-
G432004-01

Bulk CPG
500Å loading 30-40µmol/g
1g
dA(tac)
A302001-01
dC(tac)
C302001-01
dG(tac)
G302001-01
1000Å loading 25-35µmol/g
dA(tac)
A402001-01
dC(tac)
C402001-01
dG(tac)
G402001-01


2. dG(dmf) Method

DNA Phosphoramidites
PE 8900 and Polygen compatible
1gx12
2gx12
dG(dmf)
G115081-12
G115082-12
1oz
2oz
20/400 screw-cap bottle

ABI compatible
1gx12
2gx12
dG(dmf)
G115031-12
G115032-12
15ml
60ml
septum bottle

For bulk quantities please enquire.


Controlled Pore Glass
Bulk CPG
500Å loading 30-40µmol/g
1g
10g
dG(dmf)
G305001-01
G305010-01


3. Substituting the dC Protecting Group

DNA Phosphoramidites
PE8900 and Polygen compatible
1gx1
2gx1
dC(tac)
C112081-01
C112082-01
1gx12
2gx12
dC(tac)
C112081-12
C112082-12
1oz
2oz
20/400 screw-cap bottle

ABI compatible
1gx1
2gx1
dC(tac)
C112031-01
C112032-01
1gx12
2gx12
dC(tac)
C112031-12
C112032-12
15ml
60ml
septum bottle

For bulk quantities please enquire.


Controlled Pore Glass
Bulk CPG
500Å loading
30-40µmol/g
1000Å loading
25-35µmol/g
1g
1g
dC(tac)
C302001-01
dC(tac)
C402001-01


Custom Products

Please enquire for custom DNA Phosphoramidites, CPG and Columns.


Ohter quantities are available upon request.

　

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