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Description
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| Hi-Taq
is isolated and purified from an E. Coli Strain that carries the cloned
DNA polymerase gene from Thermus aquqticus YT-1 strain.
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Unit |
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500 units/vial
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Concentration |
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| 2 units/μl
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Volume |
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| 250μl/vial
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Storage
and dilution buffer |
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| 20mM
Tris-HCl(pH8.5), 100mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% Glycerol, 1% Triton
x-1000
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Stability |
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| The undiluted enzyme solution is stable when store
at -20℃
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Unit
definition |
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| One
unit is the amount of enzyme that will incorporate 10 nmoles of dNTPs into
acid-insoluble products at 72℃
in 30 minutes under the assay conditions
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Assay
conditions for unit definition
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25mM
Tris-HCl(pH8.5), 1mM B-mercaptoethanol, 10mM MgCl2, 25mM KCl, 200mM
each dGTP, dCTP, dATP,[3H]dTTP, 0.25mg /ml calf thymus DNA
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Reaction
buffer |
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| 10X-concentration
reaction buffer supplied with 17.5mM MgCl2. Total volume is 1ml/vial
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Nuclease
Assay |
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| No
nuclease activity is observed after incubation of 10u of Hi-Taq with 1mg
lambda DNA, 1mg
pGEM4Z DNA for 1 hour at 72℃No
nuclease activity is observed after incubation of 10u of Hi-Taq with 1mg
lambda DNA, 1mg
pGEM4Z DNA for 1 hour at 72℃ |
|
PCR test |
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| Good
performance of DNA amplification by polymerase chain reaction was confirmed by
using lambda DNA(amplified 10kb DNA fragment) and Human genomic DNA (amplified
0.86kb b-actin,1.32kb
b-globin,
1.26kb p53 DNA fragment) as the templates
. |
